摘要
采用原位杂交和免疫组化技术，检测２０例腮腺淋巴上皮瘤样癌（ＬＥＬＣ）组织中ＥＢ病毒编码的小ＲＮＡ（ＥＢＥＲｓ）、潜伏感染膜蛋白（ＬＭＰｌ）、溶解感染期立即早期基因编码蛋白ＺＥＢＲＡ、早期基因编码蛋白ＥＡ－Ｄ、晚期基因编码蛋白ＶＣＡ和ＭＡ。结果ＥＢＥＲｓ阳性率为１００％（２０／２０），ＬＭＰｌ阳性率为８５％（１７／２０），ＺＥ－ＢＲＡ均为阴性，ＥＡ－Ｄ阳性率８５％（１７／２０），ＶＣＡ阳性率６０％（１２／２０），ＭＡ阳性率为０．５％（１／２０）。阳性信号限于癌细胞。癌周正常组织和间质细胞均阴性。说明在我国南方鼻咽癌高发区，腮腺ＬＥＬＣ的发生发展与ＥＢ病毒感染密切相关，本文还对ＥＢ病毒基因的腮腺ＬＥＬＣ中表达的生物学意义作了初步探讨。
Twenty cases of lymphoepithelioma like carcinoma(LELC)in the parotid gland were studied for Epstein Barr virus(EBV)gene expression using in situ hybridization and immunohistochemistry.The results showed that positive signal of EBV encoded small RNAs(EBERs) could be found in the nuclei of tumor cells in 100% cases of parotid gland LELC.Latent membrane protein(LMPl) were detected on membrane and in cytoplasm of tumor cells in 85% cases.No positive staining of immediate early gene products ZEBRA were found in all cases.Early gene products EA D were positive in 85% cases.The expression rate of late gene products VCA and MA in 20 cases was 60% and 0.5% respectively.In the positive cases,the positive signals were located in the neoplastic epithelial cells,not in the normal epithelial cells and lymphoid cells.The results confirmed a consistent association between EBV and parotid gland LELC in Guangdong,a high prevalent area of nasopharyngeal carcinoma in China.The biological significance of EBV gene expression in the parotid gland LELC was discussed.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
1997年第S1期22-25,98,共5页
Journal of Sun Yat-Sen University:Medical Sciences
