摘要
目的?观察血小板衍生生长因子(PDGF)对培养的人血管平滑肌细胞增殖及钙调素含量变化的作用。方法:应用3H- TdR掺入法和磷酸二酯酶法,观察不同浓度(1、10、20、30、40ng/ml)PDGF-BB对培养的人血管平滑肌细胞DNA合成和钙调素 含量变化的影响及其时间效应。结果:不同浓度PDGF-BB作用后,处于静止状态的人血管平滑肌细胞DNA的合成增加,并呈 现明显的浓度依赖关系,在40ng/ml浓度时DNA的合成达到高峰(P<0.01)。平滑肌细胞在进入细胞增殖周期后,细胞内钙调 素含量逐渐增加,在9h时出现一个短暂的高峰,随后逐渐下降;不同浓度PDGF-BB作用后,钙调素含量增加,并呈现出明显的 浓度依赖关系,30ng/ml浓度时作用最为显著(P<0.01)。结论:PDGF可促进培养的人血管平滑肌细胞增殖,该作用可能与增 加钙调素含量有关。
Objective:To investigate the effects of platelet-derived growth factor BB(PDGF-BB)on proliferation and calmodulin content in human vascular smooth muscle cells(hVSMCs).Methods:The hVSMCs were cultured;the effects of PDGF-BB on DNA synthesis and calmodulin content in hVSMCs were assayed at different time points and different concentra-tions by 3 H-TdR incorporation and phosphodiesterase in vitro.Results:The DNA synthesis of quiescent hVSMCs was pro-moted in a dose-dependent manner,with the maximal response at a PGDF-BB concentration of40ng/ml at36h.Calmodulin level was increased after hVSMCs proliferation and reached a peak at9h.The content of calmodulin increased in a dose-de-pendent manner,with the maximal response at a PGDF-BB concentration of30ng/ml.Conclusion:PDGF-BB can promote the proliferation of hVSMCs,which may be related to the calmodulin level. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第6期620-622,共3页
Academic Journal of Second Military Medical University
基金
上海市科技发展攻关计划基金(014119072)
上海市自然科学基金(03ZR14029).
关键词
血小板衍生生长因子
诱导
血管平滑肌细胞
细胞增殖
钙调素
platelet-derived growth factor
vascular smooth muscle cells
cell proliferation
cell cycles
calmodulin
