摘要
To investigate mitochondrial DNA (mt DNA) mutations in optic neuritis of unknown cause(ONUC)and to assess the practical value of mt DNA mutation detection in etiologically and differentially diagnosing ONUC Methods Thirty patients with ONUC were screened for mt DNA mutations of nt11778, nt3460 and nt15257 by using SSCP, mutation specific primer PCR and sequencing Results mt DNA mutations were found in twelve of thirty ONUC patients All of the mutations were at nt11778 position, but no one at nt3460 and nt15257 Conclusions Forty percent (12/30) of ONUC patients were caused by an mt DNA mutation Combined with other routine measures, screening for mt DNA mutations in ONUC patients is of great significance in diagnosing ONUC etiologically and differentially
To investigate mitochondrial DNA (mt DNA) mutations in optic neuritis of unknown cause(ONUC)and to assess the practical value of mt DNA mutation detection in etiologically and differentially diagnosing ONUC Methods Thirty patients with ONUC were screened for mt DNA mutations of nt11778, nt3460 and nt15257 by using SSCP, mutation specific primer PCR and sequencing Results mt DNA mutations were found in twelve of thirty ONUC patients All of the mutations were at nt11778 position, but no one at nt3460 and nt15257 Conclusions Forty percent (12/30) of ONUC patients were caused by an mt DNA mutation Combined with other routine measures, screening for mt DNA mutations in ONUC patients is of great significance in diagnosing ONUC etiologically and differentially
基金
Thisworkwassupportedinpartbythe 86 3PlanofChina (No Z19 0 1 0 4 0 2 )
