摘要
目的 观察肝硬化患者血清和腹水激活素A浓度变化及其影响因素。方法 采用ELISA法分别测定 45例肝硬化患者 (Child PughA级 10例 ,B级 2 3例 ,C级 12例 )血清和腹水激活素A ,采用ELISA法测定 2 0名正常人血清激活素A。采用标准酶法测定血清门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶 (ALT)、碱性磷酸酶 (ALP)及尿素氮。结果 肝硬化患者血清激活素A的水平 ( 2 .71ng/ml± 1.90ng/ml)较对照组 ( 0 .2 1ng/ml± 0 .15ng/ml)明显升高 (P <0 .0 1)。Child PughA、B、C级肝硬化患者血清激活素A分别为 ( 1.44± 0 .16)ng/ml、( 1.95± 0 .45 )ng/ml和 ( 3 .0 8± 0 .65 )ng/ml,各级间比较差异有显著性 (P <0 .0 1)。血清激活素A与Child Pugh分级 (r =0 .894,P =0 .0 41)、ALT(r =0 .64 7,P =0 .0 0 0 )、AST(r =0 .5 5 0 ,P =0 .0 0 0 )及ALP(r=0 .3 2 8,P =0 .0 2 8)具有正相关性。肝硬化患者腹水激活素A水平为 ( 0 .43± 0 .2 4)ng/ml,Child PughA、B、C级肝硬化患者腹水激活素A分别为 ( 0 .3 9± 0 .2 1)ng/ml、( 0 .48± 0 .2 7)ng/ml和 ( 0 .41± 0 .2 2 )ng/ml ,各级之间比较差异有显著性 (P <0 .0 1) ;腹水激活素A与血清激活素A无相关性 ;与肾功能呈负相关 (r =-0 .415 ,P =0 .0 0 3 )。
Objective To observe the levels of serum and ascitic activin A in patients with liver cirrhosis,and investigate the possible related factors for their changes.Methods The levels of serum and ascitic fluid activin A in 45 patients with liver cirrhosis(10 Child-Pugh A,23 Child-Pugh B and 12 Child-Pugh C),and in 20 healthy controls were measured by enzyme-linked immunosorbent assays(ELISA).The levels of serum aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase and urea were detected by standard enzymatic methods. Results The serum levels of activin A in liver cirrhosis(2.71±1.90 ng/ml)were significantly higher than those in controls(0.21±0.15 ng/ml,P<0.01).There were different serum activin A levels among Child-Pugh A(1.44±0.16ng/ml),Child-Pugh B(1.95±0.45ng/ml)and Child-Pugh C(3.08±0.65ng/ml),(P<0.01).The levels of serum activin A in patients with cirrhosis positively correlated with Child-Pugh grades(r=0.894,P=0.041),aspartate aminotransferase(r=0.647,P=0.000),alanine aminotransferase(r=0.550,P=0.000) and alkaline phosphatase(r= 0.328, P=0.028).The levels of ascitic activin A in patients with cirrhosis were (0.43±0.24) ng/ml,they were different among Child-Pugh A(0.39±0.210g/ml),B(0.48±0.27ng/ml)and C(0.41± 0.22 ng/ml,P<0.01).The levels of ascitic activin A were not associated with the levels of serum activin A,but negatively correlated with the serum urea levels(r=-0.415,P=0.003).Conclusions Serum levels of activin A were elevated in patients with cirrhosis.The increase may be related to the severity of liver damage and the renal function.
出处
《临床内科杂志》
CAS
北大核心
2004年第6期400-402,共3页
Journal of Clinical Internal Medicine
基金
卫生部内科消化重点实验室开发基金资助项目 (基金编号 :WKL2 0 0 0 0 8)
