摘要
目的研究caspase-3与HIF-1α在胃癌中表达的相互关系,探讨它们在胃癌的发生发展、浸润和转移中的作用。方法应用免疫组化SP法检测54例胃癌中caspase-3、HIF-1α的表达情况,探讨caspase-3、HIF-1α与胃癌临床病理因素的关系。结果胃癌中caspase-3低表达(12/54,22.2%),且有淋巴转移(P<0.05)、临床分期高(P<0.05)、病理分化低(P<0.05)时降低。HIF-1α在胃癌中的表达率(74.07%)明显高于正常胃组织(0%),P<0.01;HIF-1α的表达与胃癌TNM分期、肿瘤浸润深度和淋巴结转移显著相关,P<0.05。胃癌组织中caspase-3与HIF-1α的表达呈负相关(r=-0.45,P<0.05)。结论caspase-3和HIF-1α在胃癌的发生发展中起重要作用,联合检测可作为判断胃癌恶性程度和预后的指标。
Objective To study the expression of caspase-3 and HIF-1α in gastric cancer and their relationship with tumor angiogenesis,clinicopathologic features and prognosis.Methods Immunohistochemical technique was used to detect the expression of caspase-3 and HIF-1α in 54 cases of gastric cancer.The relationship between the expression of caspase-3,HIF-1α and tumor angiogenesis,clinicopathologic feature of patients was analyzed.Results The expression of caspase-3 in gastric cancer was low(12/54,22.2%),and reduced with increased clinical stages(P<0.05),reduced histological differentiation(P<0.05) and occurence of lymphatic metastasis(P<0.05);The positive expression rate of HIF-1α in gastric cancer(74.07%) was significantly higher than that in normal gastric tissue(0%,P<0.01);The expression of HIF-1α in gastric cancer was significantly associated with TNM stage,invasive depth and lymph-node metastasis(P< 0.05).There was a negative correlation between expression of caspase-3 and HIF-1α(r=-0.45,P<0.05).Conclusion Caspase-3 and HIF-1α play a very important role in the development of gastric cancer,and could be factors in diagnosis of gastric cancer and estimation of prognosis.
出处
《中国病毒病杂志》
CAS
2008年第4期255-258,共4页
Chinese Journal of Viral Diseases
基金
辽宁省自然科学基金资助项目(20072109)
沈阳市自然科学基金资助项目(1071200-1-00)
