摘要
目的:观察羌活地黄汤含药血清对软骨细胞增值及RANKL mRNA(receptor activator nuclear factor kappa B ligand)表达的影响。方法:采用血清药理学和体外培养兔关节软骨细胞的方法,通过MTS/PMS系统检测羌活地黄汤含药血清对软骨细胞增殖的影响以及采用实时荧光定量PCR检测软骨细胞RANKL mRNA表达变化。结果:羌活地黄汤含药血清对体外培养的兔关节软骨细胞有促进增殖的作用,与实验对照组相比差异均有统计学意义(P<0.05),羌活地黄汤含药血清组RANKL mRNA表达量低于同一时间节点的实验对照组。结论:羌活地黄汤含药血清能促进体外培养的软骨细胞增殖,同时能抑制软骨细胞RANKL mRNA的表达。
Objective:To investigate the proliferation efficiency of Qianghuo Dihuang decoction serum on rabbit articular chondrocytes and detect the expression level of RANKL mRNA.Methods:Rabbit articular chondrocytes were cultured in serum with Qianghuo Dihuang decoction, MTS/ PMS method was applied to measure proliferation efficiency, and fluorescent quantitative PCR was used to detect the expression level of RANKL mRNA.Results:Qianghuo Dihuang Decoction serum could promote the proliferation of rabbit articular chondrocytes, which had a different compared with the control group (P<0.05). The expression of RANKL mRNA was low in Qianghuo Dihuang Decoction group compared to that of the same time point model group.Conclusion:Qianghuo Dihuang Decoction serum can promote the proliferation of rabbit articular chondrocytes cultured in vitro, inhibit the expression of RANKL mRNA.
出处
《现代生物医学进展》
CAS
2009年第24期4660-4662,共3页
Progress in Modern Biomedicine
基金
上海市基础研究重点项目资助(08JC1418800)
关键词
类风湿性关节炎
羌活地黄汤
软骨细胞
增殖
RANKL
Rheumatoid arthritis
Qianghuo Dihuang Decoction
Chondrocytes
Proliferation
Receptor activator nuclear factor kappa B ligand
