摘要
目的探讨周期素依赖性蛋白激酶5激活物结合蛋白(C53)在不同肝癌组织及肝硬化组织中的表达及其临床病理指标关系。方法应用免疫组化方法检测高分化肝细胞癌及癌旁组织5例,中低分化肝细胞癌及癌旁组织各5例,肝内胆管癌及癌旁组织各5例,轻微早期肝脏纤维化5例,并分析其表达与临床病理指标关系。结果高分化肝细胞癌常规组5例,3例强阳性,1例弱阳性,1例为阴性,癌旁组织常规组1例阳性,3例弱阳性,1例为阴性,热修复组均为阴性;中低分化肝细胞癌常规组5例,1例强阳性,3例弱阳性,1例在操作中掉片。癌旁组织常规组5例,2例弱阳性,3例为阴性;肝内胆管癌常规组5例,1例阳性,1例弱阳性,3例为阴性,而热修复组均为阳性。癌旁组织常规组1例阳性,4例为阴性,热修复组均为阴性。轻微早期肝脏纤维化组5例,C53均无表达。结论C53主要定位在肝细胞胞浆中,其表达的强弱与肝细胞癌的分化程度有关,癌旁组织也有低表达,轻微早期肝脏纤维化没有表达。
Objective To investigate the correlation between the expression and clinicopathological significance of HCC. Methods The expression of C53 was studied immtmohistochemically in well-differentiated hepatocellular carcinomas (HCC), moderately- and poorly-differentiated HCC, tumor-adjacent normal tissues and liver fibrosis at early stage. Results C53 expression was quite obvious in 3 of 5 well-differentiated HCC and 1 adjacent tissue, weakly in 1 carcinoma and 3 adjacent tissues and negative in 1 carcinoma and adjacent tissues; C53 expression was also quite obvious in 1 of 5 moderately- and poorly-differentiated, weakly in 3 carcinoma and 2 adjacent tissues and negative in 3 adjacent tissues; However, C53 expression was not efficient in 5 liver fibrosis tissues. Conclusion The immunostaining showed the C53 could exist dominantly in cytoplasm of liver cells, and expression level might be correlated to cellular differentiation of HCC. C53 was expressed in tumor-adjacent normal tissues but not in liver fibrosis at the early stage,
出处
《分子诊断与治疗杂志》
2009年第2期104-107,共4页
Journal of Molecular Diagnostics and Therapy
关键词
肝癌
免疫组织化学
C53
Hepatocellular carcinomas
Immunohistochemistry
C53 protein
