摘要
An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate (ormosil)-polyvinyl acetate (PVA) matrix onto a Prussian Blue (PB)-modified glassy carbon electrode. A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl<SUB>3</SUB>, K<SUB>3</SUB>[Fe(CN)<SUB>6</SUB>] and ethylenediamine tetraacetic acid (EDTA) under cyclic voltammetric (CV) conditions. The effects of the potential range of CV conditions, electrolyte cations, applied potential, pH, temperature and co-existing substances were investigated. The detection limit of the glucose biosensor was 8.1 μmol·L<SUP>−1</SUP> (S/N = 3) with a linear range from 20 μmol·L<SUP>−1</SUP> to 2 mmol·L<SUP>−1</SUP> (R = 0.9965). The biosensor presented a fast response and good selectivity. Additionally, excellent reproducibility and stability of the biosensor were observed.
An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate(ormosil)-polyvinyl acetate(PVA) matrix onto a Prussian Blue(PB)-modified glassy carbon electrode.A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3,K3[Fe(CN)6] and ethylenediamine tetraacetic acid(EDTA) under cyclic voltammetric(CV) conditions.The effects of the potential range of CV conditions,electrolyte cations,applied potential,pH,temperature and co-existing substances were investigated.The detection limit of the glucose biosensor was 8.1 μmol·L-1(S/N = 3) with a linear range from 20 μmol·L-1 to 2 mmol·L-1(R = 0.9965).The biosensor presented a fast response and good selectivity.Additionally,excellent reproducibility and stability of the biosensor were observed.
基金
Supported by the National High Technical Development Project (863 project) Foundation (Grant No. 2006AA09Z160)
the National Natural Science Foundation of China (Grant No. 20775064)
