摘要
目的探讨血管内皮细胞生长因子(VEGF)及生长抑制因子4(ING-4)在大肠癌中的表达及其与患者临床病理特征的关系。方法选择经手术病理证实的68例大肠癌、20例大肠腺瘤及20例正常的肠黏膜组织,采用免疫组化法检测并比较各组VEGF及ING-4表达情况,分析二者与大肠癌患者临床病理特征及生存期的关系。结果 VEGF蛋白在大肠癌组、大肠腺瘤组及正常组中的阳性表达率依次下降(P<0.05、P<0.01),ING-4蛋白在大肠癌组、大肠腺瘤组及正常组中的阳性表达率依次升高(P<0.05)。VEGF蛋白在浸润浆膜外层的大肠癌组阳性率显著高于浸润肌层及浆膜层者(P<0.05),在Dukes C、D期大肠癌患者中的阳性率高于A、B期者(P<0.05),在发生淋巴结转移的大肠癌组阳性率高于无淋巴结转移者(P<0.05);ING-4蛋白在高、中分化大肠癌组阳性率显著高于低分化组(P<0.05),在Dukes A、B期大肠癌患者中的阳性率高于C、D期(P<0.05),在发生淋巴结转移的大肠癌组阳性率高于无淋巴结转移者(P<0.05)。VEGF阳性大肠癌患者3年生存率及5年生存率均显著低于阴性患者(P<0.05),ING-4阳性大肠癌患者3年生存率及5年生存率较阴性患者无显著差异(P>0.05)。结论 VEGF高表达与大肠癌浸润程度、Dukes分期、淋巴结转移及预后有关,ING-4低表达与大肠癌分化程度、Dukes分期及淋巴结转移有关,可以作为大肠癌的术前诊断、制订手术方案的及预测转移的重要指标。
Objective To explore the expressions of vascular endothelial growth factor(VEGF) and inhibitor of growth 4(ING-4) in cancer of colon,and to investigate their correlations with clinical pathological features.Methods A total of 68 cases of cancer of colon,20 cases of colorectal adenoma and 20 cases of normal intestinal mucosa were selected.Expressions of VEGF and ING-4 were determined with immunohistochemistry,and their correlations with clinical pathological features and lifetime were analyzed.Results VEGF expression was decreased in cancer of colon,colorectal adenoma and normal intestinal mucosa in order(P<0.05,P<0.01),ING-4 expression was increased in cancer of colon,colorectal adenoma and normal intestinal mucosa in order(P<0.05).VEGF protein was significantly higher in cancer of colon invading outer serosal layer,Dukes C and D,patients with lymph node metastasis than that of muscular layer and serosal lay- er,Dukes A and B,and without lymph node metastasis(P<0.05); ING-4 protein was statistically lower in well and moderate differentiated cancer of colon,Dukes C and D,and without lymph node metastasis than that of poor differentiated cancer of colon,Dukes A and B,and with lymph node metastasis(P<0.05).The 3-year and 5-year survival rates of patients with VEGF expression were obviously lower than those of without VEGF expression(P<0.05),those of ING-4 expression showed no difference than those without ING-4 expression(P > 0.05).Conclusions VEGF high expression is correlated with invasion,Dukes stage,lymph node metastasis and prognosis of cancer of co- lon,ING-4 low expression is correlated with differentiation,Dukes stage and lymph node metastasis,both of them can serve as important in- dexes in preoperative diagnosis,making operation plan,predicting lymph node metastasis and prognosis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2014年第1期19-21,共3页
Chinese Journal of Gerontology
基金
辽宁省科技厅课题(No.2011225015)
