摘要
以1-丁基-3-甲基咪唑醋酸盐([bmim]ac)作为一种绿色溶剂用于丝素蛋白的溶解。对溶解后再生丝素蛋白分子量的分布采用SDS-PAGE凝胶电泳法测试,并研究丝素蛋白/[bmim]ac溶液稳态流变性和动态粘弹性等流变性能。结果表明:溶解后再生丝素蛋白分子量在25kDa^250kDa有较宽分布,分子量分布随溶解温度升高逐渐变宽;丝素蛋白/[bmim]ac为假塑性流体,丝素蛋白质量分数和温度对流体流变性有很大的影响:随着丝素蛋白质量分数的增加,溶液表观粘度变大;溶液非牛顿指数随质量分数及温度的增高而降低。在小振幅振荡剪切的作用下,丝素蛋白/[bmim]ac弹性模量G′和粘性模量G″随频率增加而增大,随温度增高而减小,且溶液损耗角在频率0~20Hz内下降较快。
1-butyl 3-methyl imidazole acetate([bmim]ac)was used for the dissolution of room silk fibroin(RSF)as a novel green solvent.In this paper,the molecular weight distribution of regenerated silk fibroin was researched by the method of SDS-PAGE,and RSF/ [bmim]ac steady state solution rheology and rheological properties of dynamic viscoelasticity were also studied.The results show that the molecular weight of silk fibroin is distributed within 25kDa-250kDa,and the molecular weight distribution become wider as the dissolving temperature elevates.Also,the study reveals that the silk fibroin / [bmim]ac solution is pseudoplastic fluid.The mass fraction of silk fibroin and temperature have a great impact on the fluid rheology-the apparent viscosity of solution would become larger with the increase of silk fibroin mass fraction,and the non-newtonian index of solution would be reduced as mass fraction and temperature increased.Under the action of small amplitude oscillatory shear,RSF/[bmim]ac's elastic modulus G′and viscous modulus G″would increase when the frequency increases,while decrease when the temperature increases,and the spoilage angle decreases rapidly under the frequency between 0and 20Hz.
出处
《材料科学与工程学报》
CAS
CSCD
北大核心
2014年第3期441-447,共7页
Journal of Materials Science and Engineering
