摘要
目的:研究应激反应对缺血预适应心肌细胞凋亡的影响。方法:将24只大白兔随机分4组:静脉用依托咪酯建立兔去应激动物模型作为去应激预适应组(Etom)、正常应激预适应组(IPC)、甲基强的松龙组(MP)和无预适应处理组(IR),用DNA-ladder、TUNEL染色和Hoechst染色对比4组行缺血处理后细胞凋亡程度。结果:DNA-Ladder显示条带密度IR组>Etom组>MP组>IPC组。TUNEL染色检测的凋亡率:Etom组(3.0%±0.4%)和IR组(3.8%±1.3%)与IPC组(1.7%±0.2%)比较,差异有统计学意义(P<0.05);IR组和MP组(2.3%±0.8%)比较,差异有统计学意义(P<0.05)。Hoechst染色检测的凋亡率:Etom组(7.6%±0.4%)和IR组(6.2%±1.6%)与IPC组(4.1%±0.9%)比较,差异有统计学意义(P<0.05);IR组和MP组(3.5%±0.4%)比较,差异有统计学意义(P<0.05)。结论:去应激削弱了预适应对心肌细胞凋亡的抑制作用,甲基强的松龙可抑制缺血再灌注损伤的心肌细胞凋亡。
Objective: To determine the influence of stress on myocardial apoptosis in ischemic preconditioning group(IPC). Methods: Twenty-four Japanese white rabbits were randomly divided into 4 groups(n=6): an etomidate group(the Etom group) of depressed stress established by intravenous etomidate, an IPC group, an ischemic reperfusion group(the IR group) and a methylprednisolone group(the MP group). Myocardial apoptosis was examined by DNA-laddering, in situ nick-end labeling(TUNEL) and Hoechst dyeing. Results: h e DNA ladder increased in the Etom group. h e percentage of apoptosis by TUNEL method was 1.7%±0.2% in the IPC group, 2.3%±0.8% in the MP group, 3.8%±1.3% in the IR group and 3.0%±0.4% in the Etom group. Hoechst dying was 4.1%±0.9% in the IPC group, 3.5%±0.4% in the MP group, 6.2%±1.6% in the IR group and 7.6%±0.4% in the Etom group. h ere was signii cant dif erence between the IPC group and the Etom group or IR group, and also between the MP group and the IR group. Conclusion: A depressed stress response impairs the inhibition on myocardial apoptosis in ischemic preconditioning. Methylprednisolone may inhibit myocardial apoptosis.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2014年第5期477-482,共6页
Journal of Central South University ：Medical Science
关键词
缺血预适应
去应激
心肌保护
心肌细胞凋亡
应激反应
ischemic preconditioning
depressed stress
myocardial protection
myocardial apoptosis
stress
