摘要
目的探讨DcR3在预防博莱霉素大鼠模型发生肺纤维化中的作用。方法采用博莱霉素复制肺纤维化动物模型。分为正常对照组、博莱霉素组、博莱霉素+DcR3组,给药时间2 w,各组分别于2、4、6、8 w处死大鼠6只,收集肺泡灌洗液的细胞和上清检测细胞因子含量。左肺组织冻存留作羟脯氨酸检测;右肺组织固定用作组织染色。结果博莱霉素+DcR3组肺泡炎的程度表现为逐渐减轻的过程,炎症程度均低于BL组(P<0.05);BL+DcR3组肺纤维化程度无明显加重趋势,与对照组比较差异显著(P<0.05),与BL组比较有显著性差异(P<0.01)。结论 DcR3的早期应用,可减轻博莱霉素大鼠模型肺的的局部炎症,可能在一定程度上达到预防肺纤维化形成的目的。
Objective To investigate the role of DcR 3 in preventing bleomycin rat model of pulmonary fibrosis .Methods The ble-omycin animal model of pulmonary fibrosis was established .The animal models were divided into normal control , bleomycin, bleomycin +DcR3 groups.The alveolar lavage fluid cells were cultured and supernatant , and the levels of cytokines were detected .Results The degree of alveolitis was reduced in Bleomycin+DcR3 group, which was lower than that of BL group (P<0.05);and the degree of lung fibrosis was not significantly increased trend in Bleomycin +DcR3 group,which had significant difference compared with that of BL and control groups (P<0.05, P<0.01).Conclusions DcR3 early application could reduce lung local inflammation in bleomycin rat model , achieve the pur-pose of prevention of pulmonary fibrosis in some extent .
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2014年第9期2462-2465,共4页
Chinese Journal of Gerontology
基金
国家自然科学基金项目(30370617)
关键词
DCR3
炎症
肺纤维化
DcR3
Inflammation
Pulmonary fibrosis
