摘要
AIM: To examine the α-Gal gene expression and distribution in the different species/genus and developing phase animal ocular surface tissue.METHODS: α-Gal binding assay were carried out on various animal eye sections. Photograph, slit-lamp observation on various eye showed normal corneal transparence.RESULTS: A strong α-Gal expression in invertebrates and some vertebrates ocular tissue, but no α-Gal binding in birds, fish and mammal. α-Gal expression change in the development of mice ocular surface tissue (except sclera) and display genus dependency in the different murine ocular surface tissue.CONCLUSION: This study identified specific α-Gal epitopes binding area in the ocular surface of several species and may solve the problem that naive ocular surface may be used as natural α-Gal gene knockout model/high risk immunologic rejection model or ocular surface scaffold material.
基金
National Natural Science Foundation of China (No. 81160118)
Natural Science Foundation of Jiangxi Province (No. 20114BAB215029)
Technology Foundation of Jiangxi Province (No. 20111BBG70026-2)
Health Department Science and Technology Foundation(No.20121026)
Education Department Youth Scientific Research Foundation (No. JJJ12158)
National High Technology Research of China (863 project) (No. 2006AA02A131)
