摘要
Objective:To determine the larvicidal and adult emergence inhibition activities of castor （Ricinus communis） seed extract against three potential mosquito vectors Anopheles stephensi（An. slephensi）,Culex quinquefasciatus（Cx.quinquefasciatus） and Aedes albopictus（Ae.albopictus） in India.Methods:The R.communis seed extract was tested,employing WHO procedure,against fourth larval instars of the three mosquito species for 24 h and larval mortalities were recorded at various concentrations（2-64μg/mL）:the 24 h LC<sub>50</sub> values of the R.communis seed extract were determined following Probit analysis.The larval killing,antipupation and adult emergence inhibition rates of the test extract,using a single concentration of 2μLC<sub>50</sub>,were studied at different time periods（24-72 h）:the extract toxicity was tested against a fish.Oreochromis niloticus（O.niloticus）.Results:The R.communis seed extract exhibited larvicidal effects with 100%killing activities at concentrations 32-64μg/mL,and with LC<sub>50</sub> values 7.10.11.64 and 16.84μg/mL for Cx.quinquefasciatus,An.stephensi and Ae.albopictus larvae,respectively. When the larvae were treated with the extract at a single concentration of 2×LC<sub>50</sub>,significant differences were observed,compared to control groups,in rate of pupation（P【0.001） as well as in adult formation（P【0.001）.Conclusions:The present findings suggest that the R.communis seed extract provided an excellent potential for controlling An.stephensi,Cx.quinquefasciatus and Ae. albopictus mosquito vectors.
Objective:To determine the larvicidal and adult emergence inhibition activities of castor (Ricinus communis) seed extract against three potential mosquito vectors Anopheles stephensi(An. slephensi),Culex quinquefasciatus(Cx.quinquefasciatus) and Aedes albopictus(Ae.albopictus) in India.Methods:The R.communis seed extract was tested,employing WHO procedure,against fourth larval instars of the three mosquito species for 24 h and larval mortalities were recorded at various concentrations(2-64μg/mL):the 24 h LC_(50) values of the R.communis seed extract were determined following Probit analysis.The larval killing,antipupation and adult emergence inhibition rates of the test extract,using a single concentration of 2μLC_(50),were studied at different time periods(24-72 h):the extract toxicity was tested against a fish.Oreochromis niloticus(O.niloticus).Results:The R.communis seed extract exhibited larvicidal effects with 100%killing activities at concentrations 32-64μg/mL,and with LC_(50) values 7.10.11.64 and 16.84μg/mL for Cx.quinquefasciatus,An.stephensi and Ae.albopictus larvae,respectively. When the larvae were treated with the extract at a single concentration of 2×LC_(50),significant differences were observed,compared to control groups,in rate of pupation(P<0.001) as well as in adult formation(P<0.001).Conclusions:The present findings suggest that the R.communis seed extract provided an excellent potential for controlling An.stephensi,Cx.quinquefasciatus and Ae. albopictus mosquito vectors.
