摘要
Objective:To investigate the potentials of the root bark of Annona（A.） senegalensis in the control of seizure and related hypnotic and motor incoordination effects in mice using experimental models.Methods:The methanol extract（ME） of the root bark of A.senegalensis was studied in mice using pentylenetetrazole（PTZ） induced convulsions,phenobarbitone induced sleeping time and motor coordination test on rota-rod performance.Acute toxicity and lethality（LD50） lest as well as phytochemical analysis were also carried out.Results:The extract（200,400,800 mg/ kg） exhibited a non- dose dependent significant（P【0.05） delay in the onset of both tonic and clonic phases of seizure induced by PTZ（60 mg/kg,s.c.） as well as offered a 100%protection （200 mg/kg） in mice from PTZ induced seizures.The extract significantly（P【0.05） decreased the latency and increased the duration of phenobarbitone induced sleeping time.At 200 mg/kg, the extract exhibited a significant（P【0.05） motor incoordination.The acute toxicity test revealed an oral LD<sub>50</sub> of 1 2%mg/kg,while the phytochemical studies showed the presence of alkaloids, resins,glycosides,carbohydrate,reducing sugar,flavonoids,terpenoids,saponins and tannins. Conclusion:The extract of A senegalensis possessed anticonvulsant activity with pronounced hypnotic and muscle relaxant effects.
Objective:To investigate the potentials of the root bark of Annona(A.) senegalensis in the control of seizure and related hypnotic and motor incoordination effects in mice using experimental models.Methods:The methanol extract(ME) of the root bark of A.senegalensis was studied in mice using pentylenetetrazole(PTZ) induced convulsions,phenobarbitone induced sleeping time and motor coordination test on rota-rod performance.Acute toxicity and lethality(LD50) lest as well as phytochemical analysis were also carried out.Results:The extract(200,400,800 mg/ kg) exhibited a non- dose dependent significant(P<0.05) delay in the onset of both tonic and clonic phases of seizure induced by PTZ(60 mg/kg,s.c.) as well as offered a 100%protection (200 mg/kg) in mice from PTZ induced seizures.The extract significantly(P<0.05) decreased the latency and increased the duration of phenobarbitone induced sleeping time.At 200 mg/kg, the extract exhibited a significant(P<0.05) motor incoordination.The acute toxicity test revealed an oral LD_(50) of 1 2%mg/kg,while the phytochemical studies showed the presence of alkaloids, resins,glycosides,carbohydrate,reducing sugar,flavonoids,terpenoids,saponins and tannins. Conclusion:The extract of A senegalensis possessed anticonvulsant activity with pronounced hypnotic and muscle relaxant effects.
