摘要
Objective:To find out the anti-mycobacterial potential of Cassia sophera(C.sophera),Urtica dioica(U.dioica),Momordica dioica,Tribulus terrains and Coccinia indica plants against multidrug resistant(MDR) strain of Mycobacterium tuberculosis(M.tuberculosis).Methods:Plant materials were extracted successively with solvents of increasing polarity.Solvent extracts were screened for anti-mycobacterial activity against fast growing,non-pathogenic mycobacterium strain,Mycobacterium semegmatis,by disk diffusion method.The active extracts were tested against MDR and clinical isolates of M.tuberculosis by absolute concentration and proportion methods.The active extracts were subjected to bio-autoassay on TLC followed by silica column chromatography for isolation of potential drug leads.Results:Hexane extract of U.dioica(HEUD) and methanol extract of C.sophera(MECS) produced inhibition zone of 20 mm in disc diffusion assay and MIC of 250 and 125μg/mL respectively in broth dilution assay against Mycobacterium semegmatis.Semipurified traction F2 from MECS produced 86%inhibition against clinical isolate and 60%inhibition against MDR strain of ilf.tuberculosis.F18 from HEUD produced 81%inhibition against clinical isolate and 60%inhibition against MDR strain of M.tuberculosis.Phytochemical analysis indicated that anti-mycobacterial activity of MECS may be due to presence of alkaloids or flavonoids and that of HEUD due to terpenoids.Condosions:C.sophera and U.dioica plant extracts exhibited promising anti-mycobacterial activity against MDR strain of M.tuberculosis. This is the first report of anti-mycobacterial activity form C.sophera.This study showed possibility of purifying novel anti-mycobacterial compound(s) from C.sophera and U.dioica.
Objective:To find out the anti-mycobacterial potential of Cassia sophera(C.sophera),Urtica dioica(U.dioica),Momordica dioica,Tribulus terrains and Coccinia indica plants against multidrug resistant(MDR) strain of Mycobacterium tuberculosis(M.tuberculosis).Methods:Plant materials were extracted successively with solvents of increasing polarity.Solvent extracts were screened for anti-mycobacterial activity against fast growing,non-pathogenic mycobacterium strain,Mycobacterium semegmatis,by disk diffusion method.The active extracts were tested against MDR and clinical isolates of M.tuberculosis by absolute concentration and proportion methods.The active extracts were subjected to bio-autoassay on TLC followed by silica column chromatography for isolation of potential drug leads.Results:Hexane extract of U.dioica(HEUD) and methanol extract of C.sophera(MECS) produced inhibition zone of 20 mm in disc diffusion assay and MIC of 250 and 125μg/mL respectively in broth dilution assay against Mycobacterium semegmatis.Semipurified traction F2 from MECS produced 86%inhibition against clinical isolate and 60%inhibition against MDR strain of ilf.tuberculosis.F18 from HEUD produced 81%inhibition against clinical isolate and 60%inhibition against MDR strain of M.tuberculosis.Phytochemical analysis indicated that anti-mycobacterial activity of MECS may be due to presence of alkaloids or flavonoids and that of HEUD due to terpenoids.Condosions:C.sophera and U.dioica plant extracts exhibited promising anti-mycobacterial activity against MDR strain of M.tuberculosis. This is the first report of anti-mycobacterial activity form C.sophera.This study showed possibility of purifying novel anti-mycobacterial compound(s) from C.sophera and U.dioica.
