摘要
目的制备叶黄素缓释微球并对其质量进行评价。方法采用乳化溶剂扩散法制备叶黄素缓释微球,通过正交设计试验优化制备工艺,使用光学显微镜观察微球表面形态,应用差示扫描量热法考察叶黄素在微球中的分散状态,并对所制备微球的粒径分布、载药量、包封率及释放度进行研究。结果该法制备的微球外观圆整、流动性好;粒径在60～100μm内的微球占88.5%;包封率可达(82.1±2.3)%;差示扫描量热法的分析结果显示药物以无定形状态存在于微球中。结论该法制备的微球重现性好,操作简单易行,适合于制备包封率较高的叶黄素缓释微球。
Objective To prepare and investigate the sustained release microspheres of lutein. Methods The sustained release microspheres of lutein were prepared by emulsion solvent diffusion method and factors affecting the preparation process were optimized by the orthogonal experimental design. Surface morphology of the lutein microspheres was observed by light microscope. DSC scanning method was used to check the drug’s existing state in the microspheres. The particle size distribution, drug loading, entrapment efficiency and release rate of the microspheres were also studied. The resultant microspheres were evaluated with their shape, size distribution, recovery and in vitro release in pH=6.6 phosphate solution containing 1% SDS. Results It was showed that the microspheres were spherical in their morphology. The drug existed in an amorphous state in the microspheres. The diameters of 88.5% microspheres were in the range of 60-100 μm. The highest entrapment efficiency was (82.1±2.3)%. Conclusions The method is suitable to prepare EC-lutein sustained release microspheres with high encapsulation efficiency.
出处
《中国药剂学杂志（网络版）》
2009年第5期391-397,共7页
Chinese Journal of Pharmaceutics：Online Edition
关键词
药剂学
叶黄素
缓释微球
乙基纤维素
乳化溶剂扩散法
pharmaceutics
lutein
sustained release microsphere
EC
emulsion solvent diffusion method
