摘要
目的比较哨点监测中常用的两种梅毒血清学实验室检测方法的敏感性和特异性。方法采用酶联免疫试验(ELISA)及梅毒甲苯胺红不加热血清试验(TRUST)法,同时筛查801份哨点监测标本中的梅毒指标,结果采用梅毒螺旋体明胶凝集实验(TPPA)法进行比对。结果在TPPA法确证的92例阳性标本中,TRUST检出53例,灵敏度57.61%;ELISA检出90例,灵敏度为97.83%。TRUST检出的55例阳性中,有2例经确证为阴性,特异性为96.36%;ELISA检出的91例阳性中,有1例经确证为阴性,特异性为98.90%。结论 ELISA试剂的灵敏度与特异性均高于TRUST试剂,与TPPA符合率高,用两种试剂组合来进行哨点监测,可以用较低的费用达到良好的监测效果。
Objective To compare the sensitivity and specificity of two common syphilis serology laboratory tests in sentinel surveillance.Methods 801 specimens were screened in terms of syphilis indicators in sentinel surveillance by using ELISA and TRUST methods.The results were compared with TPPA method.Results In the 92 cases confirmed positive samples by TPPA,53 cases were detected by TRUST method,with the sensitivity 57.61%,while 90 cases were detected by ELISA method,with the sensitivity97.83%.TRUST detected 55 cases positive,and two cases were confirmed as negative.The specificity was96.36%.ELISA detected 91 cases positive,and one case was confirmed as negative.The specificity was 98.90%.Conclusion The sensitivity and the specificity of ELISA kits were higher than those of TRUST reagents.ELISA has a high accordance rate with TPPA.Sentinel surveillance with the combination of two reagents can achieve good monitoring results,which is effective and lower in cost.
出处
《健康教育与健康促进》
2013年第5期384-385,397,共3页
Health Education and Health Promotion
