摘要
目的观察肝门阻断再灌注(HPO)后肠黏膜的损伤并探讨可能机制。方法 16只大鼠随机分成Sham组和HPO组,分别建立假手术对照、肝门阻断再灌注模型,以肠黏膜为目标,采用干湿重法比较肠壁组织含水率、光镜研究组织病理、Chiu'评分法评价肠黏膜损伤、髓过氧化酶(MPO)测定中性粒细胞(PMN)浸润程度、放免法测定血浆TNF-α浓度以及ELISA法测定血浆细胞间黏附分子1(ICAM-1)浓度等。观察HPO再灌注后致肠黏膜损伤中主要细胞因子的作用。结果 (1)HPO组见明显肠黏膜损伤,Chiu'评分312±42远大于Sham组的30±11(P<0.01);再灌注后肠MPO活性明显增高为(0.99±0.25)ΔOD·g-1·min-1,高于Sham组(P<0.05)。(2)和Sham组[(77.3±1.1)%]比较,HPO组肠组织含水量明显增高[(81.1±1.8)%,P<0.05]。(3)和Sham组比较,HPO组TNF-α和ICAM-1浓度升高明显,分别为(1.2±0.2)ng/mlvs.(3.3±0.1)ng/ml和(211.1±57.1)ng/mlvs.(408.3±44.3)ng/ml(均P<0.01)。结论 HPO后肠淤血再灌注损伤严重,致炎因子TNF-α大量生成引起PMN激活和聚集,ICAM-1介导了PMN细胞毒效应可致肠黏膜屏障损伤。
Objective To observe the damages of intestinal mucosa undergone hepatic portal occlusion(HPO)in vivo and explored the mechanisms of TNF-α and ICAM-1.Methods In the research,the Pringle′ measure was used to install HPO model without bypass in rats and observed the subsequent intestinal mucosa pathological changes and permeability.The morphology were studied by light microscope,MPO measurement test PMN infiltration level in intestine,and also test the concentration of TNF-α(ELISA)and ICAM-1(radioimmunoassay)in plasma.Results (1)The Chiu′ scores in Group HPO,Group Sham were 312±42 vs.30±11 respectively.The intestine PMN infiltration in Group HPO were more serious than Group Sham.(2)The water ratio of tissue in Group HPO were increased and show intestinal mucosa edema tendency.(3)The concentration of TNF-α,ICAM-1 in Group HPO were obviously higher than those in Group Sham(all P<0.05).Conclusions Intestine were seriously injuried undergone HPO reperfusion,and the concentration of plasma TNF-α and ICAM-1 increased obviously and play role to promote PMN infiltration.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第19期5873-5876,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
福建省自然科学基金(2008J0265)
福建省卫生厅医学创新基金(2009-CBX-3)
