摘要
通过紫外-可见吸收光谱法和荧光光谱法在近生理条件下研究双酚A对牛血清白蛋白的毒性作用。实验结果表明,双酚A溶液对牛血清白蛋白有荧光猝灭作用,猝灭机理为静态猝灭,二者的结合常数为2.541 61×103,结合位点数为0.655 931。紫外可见光谱的结果说明双酚A可以使牛血清白蛋白的结构发生变化从而显示其毒性效应。
The toxic interaction of bisphenol A(BPA) with bovine serum albumin(BSA) was studied by using ?uorescence spectra and UV-vis absorption spectra.The experimental results indicate that the intrinsic fluorescence of BSA can be quenched by the addition of bisphenol A.The quenching mechanism is due to static quenching,and the binding constant is 2.54161×103,and the number of binding sites is 0.655931.The experimental results also show that the binding of bisphenol A to BSA can induce conformational changes to BSA,as shown by the UV–vis spectra.Such conformation changes can induce toxic effects.
出处
《辽宁化工》
CAS
2012年第12期1245-1247,共3页
Liaoning Chemical Industry
