摘要
目的 探讨多重耐药大肠埃希菌中7种相关毒力基因存在情况.方法 用pcr方法检测多重耐药大肠埃希菌中7种毒力基因(papa、cnf1、cnf2、cfab、ipab、hofq和ompt),并研究pcr阳性基因在biocyc基因组数据库31株已完成全基因组测序的大肠埃希菌中的分布.结果 20株多重耐药大肠埃希菌中仅检测到hofq、ompt两种毒力基因,检出率分别为95.0％ (19/20)和55.0％( 11/20).biocyc基因组数据库的31株大肠埃希菌中,21株存在hofq基因,阳性率为67.7％；15株存在ompt基因,阳性率为48.4％.结论 多重耐药大肠埃希菌中毒力基因hofq和ompt的携带率较高.
objective to investigate the distribution of virulence-related genes in multidrug resistant escherichia coli.methods seven virulence genes papa,cnf1,cnf2,cfab,ipab,hofq and ompt were detected by pcr in 20 strains of multidrug resistant escherichia coli clinically isolated,and the positive genes were further searched in 31 strains of escherichia coli in biocyc database whose genomies had been fully sequenced.results virulence genes hofq and ompt were detected in 20 strains of escherichia coli with a positive rate of 95.0％ (19/20) and 55.0％ ( 11/20),respectively.among 31 strains of escherichia coli in biocyc,21 (67.7％) were positive for hofq gene and 15 (48.4％) were positive for ompt gene.conclusion hofq and ompt genes are prevalent in multidrug resistant escherichia coli.
出处
《中华临床感染病杂志》
CAS
2012年第1期19-23,23,后插1－后插2,共5页
Chinese Journal of Clinical Infectious Diseases
基金
宁波市自然科学基金资助项目( 2010A610029)
