摘要
目的对血管内皮生长因子-C(VEGF-C)、血管内皮生长因子-D(VEGF-D)在非小细胞肺癌(NSCLC)组织中的表达进行研究,探讨VEGF-C、VEGF-D在NSCLC淋巴管生成和淋巴结早期转移中的作用。方法收集NSCLC患者术后肺组织标本52例,应用半定量逆转录聚合酶链反应法(RT-PCR)检测肺癌组织VEGF-C、VEGF-DmRNA的表达量。结果 (1)VEGF-CmRNA的表达水平在淋巴结转移阳性组的肺癌组织中较淋巴结转移阴性组的肺癌组织中增高(P<0.001),(2)VEGF-DmRNA的表达水平在淋巴结转移阳性组的肺癌组织中与淋巴结转移阴性组的肺癌组织中无差别。(3)VEGF-C/VEGF-D在淋巴结转移阳性组的肺癌组织较淋巴结转移阴性组的肺癌组织的值为高(P<0.05)。结论 VEGF-CmRNA的表达水平,VEGF-C/VEGF-D比值与肺癌淋巴结转移密切相关,VEGF-C可能成为判断淋巴结转移的一个重要指标。
Objective Vascular endothelial growth factor(VEGF-C,VEGF-D)is presumed to correlate with lymphangiogenesis.The purpose of this study was to identify the expression level of VEGF-C and VEGF-D in non-small-cell-lung-cancer(NSCLC)tissue,and clarify whether there is,correlation between elevated VEGF-C or VEGF-D transcription level with lymph node metastasis.Methods Lung tissues specimen were collected from 52 NSCLC patients who underwent operation,A semiquantitative multiplex RT-PCR technology was applied to measure the mRNA quantity of VEGF-C and VEGF-D in carcinoma tissue.Results(1)The VEGF-C mRNA level in lung tumor tissue was significantly higher in patient group with lymph node metastasis than that in the group without lymph node metastasis(t=4.333,P<0.001).(2)No difference was manifested between the two groups when VEGF-D mRNA level was concerned.(3)The ratio of VEGF-C/VEGF-D was significantly higher in patient group with lymph node metastasis than that without lymph node metastasis.Conclusions VEGF-C transcription levels and the ratio of VEGF-C/VEGF-D may correlate with lymph node metastasis in NSCLC patients,and might be useful predictors for lymph node metastasis in NSCLC.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第13期3763-3767,共5页
Chinese Journal of Clinicians(Electronic Edition)
关键词
癌
非小细胞肺
血管内皮生长因子C
血管内皮生长因子D
逆转录聚合酶链反应
淋巴转移
Carcinoma,non-small-cell lung
Vascular endothelial growth factor C
Vascular endothelial growth factor D
Reverse transcriptase polymerase chain reaction
Lymphatic metastasis
