摘要
目的研究RhoA在人脑胶质瘤组织中的表达情况及其与病理分级的相关性,探讨RhoA在胶质瘤的诊断、恶性程度及预后判断中的临床意义。方法采用SABC免疫组化法、RT-PCR法对53例不同病理级别的星形细胞瘤及9例正常脑组织标本,进行RhoA蛋白和RhoA mRNA的检测。结果 (1)免疫组化结果显示53例胶质瘤组织中RhoA蛋白表达全部呈阳性,对照组正常脑组织存在弱表达或不表达。(2)不同级别星形细胞瘤标本的RhoA阳性细胞率、RhoA mRNA与GAPDH mRNA灰度比值均存在差异;Ⅰ级和Ⅲ、Ⅳ级之间差异有统计学意义(P<0.05),Ⅲ、Ⅳ级RhoAmRNA表达均高于Ⅰ级;Ⅱ级和Ⅲ、Ⅳ级之间差异有统计学意义(P<0.05),Ⅲ、Ⅳ级RhoAmRNA表达均高于Ⅱ级。(3)相关性分析:RhoA阳性细胞率和RhoA mRNA表达量与胶质瘤病理分级均呈正相关性(r=0.875,r=0.817,P<0.001)。结论 (1)RhoA在所有已检测的胶质瘤标本中均存在表达,且表达程度与胶质瘤病理分级呈正相关。检测RhoA的mRNA和(或)蛋白的表达,可以作为胶质瘤诊断、判断预后的一个可靠指标。(2)RhoA表达水平与肿瘤恶性程度呈正相关,提示我们可以将其作为星形细胞瘤Ⅰ～Ⅳ级新的有效的诊断标记物,同时作为常规组织病理学的分级标准的补充。这将有助于描述肿瘤的生物学行为特征,选择术后进一步的治疗方案。
Objective To investigate the expression of RhoA in human glioma and the correlation between RhoA's expression and pathological grade,futhermore,whose clinical significance would be discussed for diagnosing the glioma,judging the malignant grades and the patients' prognosis.Methods The expression of RhoA protein and RhoA mRNA were detected in 53 cases of human glioma samples and 9 cases of normal brain tissues by SABC immunohistochemical staining and reverse transcription polymerase chain reaction(RT-PCR),respectively.Results (1)The immunohistochemistry showed that the ratio of RhoA positive cells was 100% in all detected 53 human glioma samples while there was no expression in 9 normal brain tissue samples.(2)The ratio of RhoA positive cells and the gray degree ratios of the RhoA mRNA belt relative to the GAPDH mRNA belt varied accordingly to different pathologic grades.There were differences between gradeⅠand grade Ⅲ or grade Ⅳ(P<0.05),also existed between gradeⅡ and gradeⅢ or grade Ⅳ (P<0.05).The expressions of RhoA mRNA in grade Ⅲ or grade Ⅳ were significantly more than those of gradeⅠ or gradeⅡ.(3)Analyses of correlation:The positive correlation existed between the ratio of RhoA positive cells or RhoA mRNA and the pathologic grading of the human glioma(r=0.875,r=0.817,P<0.001).Conclusions (1)RhoA was present in all 53 cases glioma samples,whose expression was correlate to grade positively.Detecting RhoA in the human glioma can be used for diagnosis,judging the glioma malignancy degree and the patients' prognosis.(2)Our findings suggested that RhoA may serve as novel and efficient diagnostic markers for astrocytic brain tumors of histological grade Ⅰ to Ⅳ and complement for currently applied histopathological analysis.It will contribute to characterizing tumors biological behavior and choosing therapeutic approach following surgery.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第9期2549-2553,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
山西省卫生厅科技攻关项目(20100224)
