摘要
目的评价FISH用于诊断上泌尿系尿路上皮癌(UUTUC)的临床应用价值。方法应用荧光原位杂交(FISH)检查和尿细胞学检查58例UUTUC患者和20例健康人群尿沉渣标本,并比较两者的效果。FISH探针采用随机引物法标记3、7、17号染色体着丝粒及9p21(p16)区带。分析FISH结果中各个探针的出现频率。结果 FISH对于UUT检查敏感性高于尿细胞学检查(75.9%vs.34.5%,P=0.0001),特异性相似(90%vs.95%,P=0.548)。FISH和尿细胞学检查阳性预测值95.6%和95.2%,阴性预测值分别是56.3%和33.3%。探针中3、7和17号染色体非整倍性扩增率分别为52.3%、56.9%和34.1%,p16杂合性缺失丢失27.3%。结论应用3、7、9和p16探针的FISH检查用于UUTUC中敏感性更高而特异性相似,可用于UUTUC的术前诊断。
Objective The purpose of this study was to evaluate the clinical utility of fluorescence in situ hybridization (FISH) assay as a non-invasive method for diagnosing urothelial carcinoma (UC) in the upper urinary tract (UUT).Methods Urine specimens from 58 consecutive patients with UUT-UC and 20 healthy controls were analyzed by cytology and FISH.For FISH analysis,labeled probes specific for chromosomes 3,7,and 17 and for the p16 (9p21) genes were used to assess chromosomal abnormalities indicative of malignancy.Sensitivity and specificity of both techniques were determined and compared.The frequency of chromosomal aberrations of malignant cells from UUT was also determined.Results Overall sensitivity of FISH was significantly higher than that of urine cytology (75.9%vs. 34.5%,P= 0.0001).Specificities for both FISH and cytology were similar results (90% vs. 95%,P=0.548).The positive and negative predictive values for FISH were 95.6% and 56.3%,whereas for cytology they were 95.2% and 33.3%,respectively.Of 58 patients with UUT UC,polysomies of chromosome 3,7 and 17 were observed in 52.3%,56.9% and 35.7%,respectively,and loss of the p16 gene in 27.3%.Conclusions FISH assay of chromosomes 3,7,9,and 17 performed on exfoliated cells from voided urine specimens has greater sensitivity than cytology for detecting UUT UC,and they were maintaining a similar specificity.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第6期1579-1582,共4页
Chinese Journal of Clinicians(Electronic Edition)
关键词
泌尿系肿瘤
原位杂交
荧光
细胞诊断学
Urologic neoplasms
In situ hybridization,fluorescence
Cytodiagnosis
