摘要
应用刺探电位图谱(EPG)技术研究了在液态人工饲料中褐飞虱(Nilaparvata lugens)口针的刺探行为,对出现的波形进行了定义,从各波形占总时间的比率、各波形的平均时间、能够产生N2波和E波的个体比例等方面进一步研究和分析了褐飞虱在不同质量浓度苦杏仁酸-蔗糖取食液中的取食行为,并应用铷元素标记法验证了EPG结果.结果表明,褐飞虱取食液态人工饲料主要出现5种波形,即N1波、N2波、N3波、E波以及非刺探波np波.在苦杏仁酸质量浓度为1.0 mg/ml时,褐飞虱取食的路径波(N2波)持续时间由空白对照的22.85 min显著延长到64.65 min,占总时间比率由对照组的10%上升至25%,而取食波(E波)缩短为9.75 min,占总时间比率由对照组的37%下降至4%,E波出现的频率由对照组100%下降至42%.苦杏仁酸对褐飞虱具有一定的拒食效果,随苦杏仁酸质量浓度的升高拒食效果更为明显,铷元素标记法测定的结果与EPG检测的E波结果基本一致,证实了EPG检测结果的可靠性.
The probing behavior of the brown planthopper(BPH,Nilaparvata lugens) feeding on different concentrations of mandelic acid(MA) was studied by the electrical penetration graph(EPG) technique and different waveforms were identified.Then studied the feeding behavior of BPH on different concentrations of MA including the percentage of different waveforms,mean duration of each waveform from BPH feeding on different concentrations of MA,and the proportions of BPH performed N2 and E waveforms.The rare element rubidium(Rb) was used to label the artificial food to detect the effect of mandelic acid on BPH.Results showed that five types of EPG waveforms performed by BPH were identified,that is,N1,N2,N3,E,and np(not probing) waveform.When the concentration of MA raised to 1.0 mg/ml,the pathway waveform(N2) reach to 64.65 min(control 22.85 min),from 10% to 25%,while the active ingestion waveform(E) decreased to 9.75 min,from 37% to 4%,and the occurrence of waveform(E) decreases from 100% to 42%.The results indicates that MA has an antifeedant effect on BPH,and with the increase of MA,the antifeedant effect will be more obvious.It is reliabe that the direct labeling of Rb experiments confirmed with the EPG results.
出处
《中国计量学院学报》
2011年第2期95-101,共7页
Journal of China Jiliang University
基金
国家自然科学基金资助项目(No.30900944)
浙江省自然科学基金资助项目(No.Z3100594
No.Y3090184
No.Y3080410)
浙江省教育厅科研项目(No.Y200805875)
