摘要
目的观察糖尿病对大鼠脊髓组织紧密连接Occludin蛋白表达的影响与不同病程阶段其变化趋势,从分子水平探讨糖尿病对血-脊髓屏障(BSCB)损害的机制。方法以链脲佐菌素介导的糖尿病大鼠模型的脊髓组织为研究对象,采用免疫组织化学方法、荧光定量PCR反应和Western Blot方法测定紧密连接Occludin蛋白的表达。结果糖尿病模型1个月组,Occludin蛋白的mRNA水平较正常组显著降低(P<0.05),糖尿病模型3个月组,其值较正常组进一步降低(P<0.01)。糖尿病模型1个月组,Occludin的蛋白质水平较正常组显著降低(P<0.05),糖尿病模型3个月组,其值较正常组降低(P<0.01)。结论糖尿病可以使BSCB紧密连接Occludin蛋白含量降低,随着病程延长其含量有逐渐降低的趋势,导致了糖尿病BSCB的损害。
Objective To observe the change of tight junction protein Occludin on blood-spinal cord barrier(BSCB) in diabetic rats,and investigate the tendency during different diabetic stages,which indicated the possible mechanism of diabetic myelopathy by molecular level.Methods The spinal cord of streptozotocin induced diabetic rats were used as research target.Immuno-histochemistry,real time RT-PCR and Western blot were applyed to evaluate the expression of tight junction protein Occludin.Results Compared with cont...
出处
《脑与神经疾病杂志》
2011年第2期96-99,共4页
Journal of Brain and Nervous Diseases
基金
上海市闵行区自然科学基金(2009106ZR27)
关键词
糖尿病
血-脊髓屏障
紧密连接蛋白
Diabetes
Blood-spinal cord barrier
Tight junction protein
