摘要
研究建立反相高效液相色谱法测定苦丁茶中熊果酸和齐墩果酸的含量。采用甲醇溶剂超声提取法,色谱条件:EclipseXDB-C18柱(5μm,4.6×150mm)、流动相为甲醇-0.2%磷酸水溶液(88:12)、检测波长为220nm、流速为0.8ml/min、柱温为35℃、进样量20μl。在此色谱条件下,熊果酸和齐墩果酸与其它成分达到了基线分离,且分别在0.204～2.04μg、0.0717～1.342μg范围内,进样量与峰面积呈良好的线性关系,二者加标回收率分别为97.65%、97.42%,RSD分别为1.96%、1.5%。本方法简便,灵敏,重现性好,结果准确可靠。
The determination of ursolic acid(UA) and oleanolic acid(OA) in Ilex kudingcha C.J.Tseng with reversed -phase high performance liquid chromatography(RP - HPLC) was developed.To extract UA and OA by supersonic technology,the chromatographic conditions were as follows;Eclipse XDB -C18 column(5μm,4.6mm×150mm),elution influent methanol -0.2%aqueous solution of phosphoric acid(88:12),detection wavelength 210 nm,flow rate 0.8ml/min,column temperature 35℃and injection volume 20μl.Under the optimal conditions, a ba...
出处
《贵州科学》
2010年第2期41-44,共4页
Guizhou Science
基金
国家科技支撑计划项目子课题(子课题编号:2007BAD76B05-02)
