摘要
为了获得稳定表达绿色荧光蛋白(GFP)的我国代表性小麦禾谷镰刀菌菌株用于小麦抗赤霉病接种鉴定与分析,以禾谷镰刀菌武昌菌株Fg175原生质体为受体,经PEG介导将序列优化的水母GFP基因转入该菌株中,分析鉴定了转基因菌株GFP基因的表达。PCR分析表明,Fg175基因组中整合了GFP基因,Western blot检测到高效表达的GFP特异蛋白;激光共聚焦显微镜及体视显微镜观察进一步证实,转基因菌株Fg175-GFP的分生孢子、菌丝及接种小麦麦穗均可发出清晰的绿色荧光。
To obtain a representative Fusarium head blight(FHB) strain from wheat in China that can stably express a green fluorescence protein and be used for FHB evaluation and analysis,a Fusarium graminearum strain,Fg175,isolated from wheat spike in Wuchang,China was subjected to generation of transgenic strains.Protoplasts from this strain were prepared and used for PEG-mediated genetic transformation with a vector carrying a codon-optimized GFP sequence.The transgenic F.graminearum strains were generated and the ...
出处
《麦类作物学报》
CAS
CSCD
北大核心
2010年第5期824-828,共5页
Journal of Triticeae Crops
基金
国家"973"项目(2009CB118806)
国家"863"高技术研究发展专项项目(2007AA10Z425)
关键词
小麦
禾谷镰刀菌
真菌原生质体
绿色荧光蛋白
荧光图像
Wheat
Fusarium graminearum
Fungal protoplast
Green fluorescence protein
Fluorescence image
