摘要
通过Reed-Muench法检测从江西发生持续高热的病猪体内分离的猪繁殖与呼吸综合征病毒(PRRSV)Nsp2基因缺失株Jiangxi-3第9代(Jiangxi-3F9)的病毒滴度;将此毒株接种于PRRSV和猪圆环病毒2型(PCV2)抗原与抗体均为阴性的60日龄健康猪进行猪半数致死量(LD50)的测定,并且对LD50试验中存活下来的试验猪再次进行较大剂量的攻毒试验,另将病毒接种于PRRSV和PCV2抗原和抗体均为阴性的6月龄健康猪,对所有攻毒试验猪通过体温检测、临床症状观察、RT-PCR、ELISA等方法进一步研究Jiangxi-3F9的毒力。结果表明:Jiangxi-3F9的病毒滴度为105.25TCID50·mL-1,Jiangxi-3F9病毒的LD50为102.·2mL-1。动物试验各项检测结果说明Jiangxi-3F9毒力强。
The virulence of Nsp2 gene deletion Jiangxi-3 of PRRSV was further studied,which was isolated from pig of the so-called"Mystery Fever Disease Syndrome"in pig herds in Jiangxi Province in China.TCID50of the 9th generation of Jiangxi-3(Jiangxi-3 F9) was detected by Reed-Muench methond.The healthy pigs which were negative of antibody and antigen of PCV2 and PRRSV were divided into 3 groups for determining LD50,and the survival pigs were challenged again.The healthy 6-month-old pigs were challenged,which were n...
出处
《上海交通大学学报（农业科学版）》
2010年第1期46-52,共7页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
国家食品安全重大专项(2001BA804A31)
广东省农业科技攻关(2003B21404
20052490100和2006B20301013)
广东省自然科学基金(04002083)
广东省自然基金重点项目(06105311)
广东省农业科学院重大专项
