摘要
目的研究蛋白激酶CK2表达沉默对鼻咽癌细胞放射增敏作用,并探讨其可能机制。方法通过RNA干扰技术下调鼻咽癌5-8F细胞中CK2α蛋白表达,利用Western blotting方法验证干扰效果;利用克隆形成实验观察CK2表达沉默后对鼻咽癌细胞放射敏感性的影响;应用免疫荧光方法检测γ-H2AX灶点形成;Annexin-V和PI双染流式细胞仪检测细胞凋亡。结果通过RNA干扰技术可以有效沉默CK2α表达。克隆形成实验结果显示CK2α表达沉默后,鼻咽癌细胞形成克隆能力明显下降,对X线的敏感性明显增加;1Gy照射后15min,CK2α沉默的细胞γ-H2AX灶点数目明显增加;CK2α沉默的细胞凋亡率明显高于对照组(P<0.01)。结论蛋白激酶CK2与鼻咽癌放射敏感性密切相关,CK2可能是一个非常有潜力的鼻咽癌治疗靶点。
Objective To investigate the effect of protein kinase CK2 gene silencing on the radiosensitization in human nasopharyngeal carcinoma (NPC) cells and its possible mechanism. Methods RNA interference (RNAi) technique was used to down-regulate the protein kinase CK2α expression in 5-8F cells, and clonogenic assay was employed to observe the changes in the radiosensitivity of the cells. DNA double-strand break was assessed by immunofluorescence staining of γ-H2AX foci, and the cell apoptosis was examined using ...
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第8期1551-1553,1556,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30672312)
关键词
蛋白激酶CK2
鼻咽肿瘤
放射增敏
沉默
RNA干扰
protein kinase CK2
nasopharyngeal carcinoma
radiosensitivity
silencing
RNA interference
