摘要
目的探讨血小板膜P选择素的表达率及血清P选择素的浓度在急性冠脉综合征(acute coronary syndrome,ACS)中的临床意义。方法采用间接免疫荧光流式细胞术和双抗夹心酶联免疫测定法分别检测30例ACS患者、20例稳定型心绞痛(stable angina,SA)患者和25例正常对照者(经冠状动脉造影检查提示冠状动脉正常)血小板膜P选择素表达率和血清P选择素浓度,并进行统计分析。结果ACS组血小板膜P选择素表达率显著高于SA组[(17.19±8.05)%vs.(13.09±6.25)%,P<0.05],并显著高于对照组[(17.19±8.05)%vs.(10.83±5.76)%,P<0.05],SA组和对照组之间比较,差异无统计学意义(P>0.05)。ACS组血清P-选择素的浓度显著高于SA组[(43.98±14.71)ng/mlvs.(36.14±13.42)ng/ml,P<0.05],并显著高于对照组[(43.98±14.71)ng/mlvs.(33.34±11.05)ng/ml,P<0.05],SA组和对照组比较,差异无统计学意义(P>0.05)。冠状动脉性心脏病(冠心病)患者血小板膜P选择素表达率与血清P-选择素浓度呈正相关(r=0.295,P=0.017)。结论检测血清P选择素浓度对预测ACS发生、发展有重要的参考价值。
Objectives To investigate the clinical value of the expression rate of P-selectin on the platelet membrance and the concentration of serum P-selectin in patients with acute coronary syndrome (ACS). Methods The expression rate of P-selectin on the platelet membrane and the concentration of serum P-selectin in 30 ACS patients, 20 stable angina ( SA) patients and 25 healthy subjects were measured by flow cytometry ( FCM) and enzyme linked immunosorbent assay (ELISA). Results The expression rate of P-selectin on the platelet membrane in ACS group was significantly higher than those in SA group and the control group [(17.19±8.05) % vs. (13.09±6.25) %, P<0.05; (17.19±8.05) % vs. (10.83±5.76) %, P<0.05], while there was no significant difference between SA group and control group (P>0.05). The concentration of serum P-selectin in ACS group was significantly higher than those in SA group and the control group [( 43.98 ±14.71) ng / mL vs. ( 36.14 ±13.42) ng / mL, P <0.05; ( 43.98 ±14.71) ng / mL vs. (33.34±11.05) ng / mL, P<0.05], while there was no significant difference between SA group and control group (P> 0.05). The expression rate of P-selectin on the platelet membrane in patients with coronary heart disease was positively correlated to the concentration of serum P-selectin (r=0.295, P=0.017). Conclusions The serum P-selectin level may be a valuable marker for predicting the development of ACS.
出处
《岭南心血管病杂志》
2009年第5期363-365,375,共4页
South China Journal of Cardiovascular Diseases
