摘要
目的:建立测定人血浆中氯氮平高效液相色谱法。方法:血浆样品经适当处理,色谱柱为Luna C18(250×4.6mm,5μm)柱,流动相为乙腈:30mmol/L醋酸铵(46:54,V/V),柱温40℃,流速0.9mL/min,检测波长243nm,艾司唑仑为内标。结果:测定方法在0.05-1.6μg/mL范围内线性良好(r=0.9992,n=6),高、中、低浓度加样回收率均在98.8%~104.0%之间,日内、日间RSD在2.96%~11.5%之间,最低检测浓度为0.01μg/mL(S/N=3)。结论:本方法快速、简便、准确,适用于临床氯氮平浓度监测。
Objective To establish a simple,sensitive and rapid HPLC method for determination of clozapine in human plasma.Methods Clozapine and was extracted from plasma with ether,chromatographic analysis was carried on a Luna C18(250×4.6mm,5μm) column and the column temperature was 40℃.The mobile phase consists of Acetonitrile-Ammonium acetate(46:54,V/V),the flow rate was 0.9 mL/min,UV detector was set at 243nm,and Estazolam was used as the internal standard.Results A linearity was obtained in the range of 0.05~1.60...
出处
《湖南师范大学学报（医学版）》
2008年第3期31-33,共3页
Journal of Hunan Normal University(Medical Sciences)
