摘要
目的 探讨蚊虫抗药性敏感测试方法,方法 采用微板法,对室内常规饲养的敏感品系和室内选育的DDVP、抗残杀威品系淡色库蚊非特异性酯酶的活性进行了测定。结果 敏感品系蚊虫OD值≥0.9的占17.16％(35/204条);<0.9的占82.84％(169/204条);抗DDVP品系OD值≥0.9的占83.33％(200/240条);<0.9的占16.67％(40/240条);抗残杀威品系≥0.9的占74.89％(176/235条),<0.9的占25.11％(59/235条)。结论 敏感品系与抗性品系淡色库蚊的平均OD值存在较大差异。
Microplate assay was employed for the determination of nonspecific esterase level in individual susceptible, anti-DDVP and anti-propoxur resistant Culex pipiens. Among susceptible mosquitoes detected, 17. 16% was of high esterase activity with OD value more than 0. 9. Among anti-DDVP and antipropoxur resistant mosquitoes, the OD value more than 0. 9 was found in 83. 33% and 74. 89% respectively. This indicated that there existed obvious difference in the level of nonspecific esterase between susceptible and resistant mosquitoes.
出处
《医学动物防制》
2001年第3期121-123,共3页
Journal of Medical Pest Control
基金
山东省卫生厅
医科院资助项目
关键词
微板法
蚊虫
非特异性酯酶
Microplate Mosquito Non-specific esterase Insecticide resistance
