摘要
目的探讨凋亡相关基因Bcl－2和Bax在增生性瘢痕（HS）中的表达水平和分布特征及其与瘢痕增生和退化关系。方法检测HS中Bcl－2和Bax的表达。结果正常皮肤不表达Bcl－2，在HS中Bcl－2和Bax的阳性率为100％。浅层Bcl－2阳性细胞率高达64％～78％，显著高于其他时间点标本和同一标本的中层和深层（P< 0.01），仅成纤维细胞表达阳性；Bax在瘢痕增生的全过程和各层组织均有较高的阳性细胞率（52％～69％）和广泛的细胞分布，显著高于正常皮肤（P< 0.01）。结论深度烧伤创面愈合后，浅层成纤维细胞过度表达Bcl－2可能与创伤修复失控和HS形成有关，而广泛的Bax蛋白的过度表达可能具有抵抗Bcl－2活性作用，加速细胞的生理性凋亡和瘢痕成熟。
：Objective To study the expression and histological distributive characteration of apoptosis－related gene Bcl－2 and Bax in hypertrophic scars (HS) and the relationship to hypertrophia and degeneration of scars. Method Detect the expression of Bcl－2 gene and Bax gene in HS.Result There was no expression of Bcl－2 in normal skin ,and the positive ratio of Bcl－2 and Bax were 100％in HS.The positive ratio of Bcl－2 was 64％～78％in superficial layer, and significantly higher than other specimen groups of different times, and higher than the middle layer and the deep layer of the same specimen group(P< 0.01).The immunoreactive positive cells were fibroblasts. The positive ratio of Bax was significantly higher than the normal skin (P< 0.01)and in the whole course of hypertrophic scars(52％～69％), and the immunoreactive positive cells of Bax were distributed extensively.Conclusion The overexpression of Bcl－2 in fibroblast might be related to out of control of wound healing and the formation of HS after the wound healing of deeper－partial burns.The extensive overexpression of Bax protein might be the active resistance effect of Bcl－2 accelerating physiological apoptosis of cells and maturation of scars.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2001年第20期68-69,共2页
Journal of Clinical Rehabilitative Tissue Engineering Research
关键词
增生性瘢痕
凋亡
基因
：hypertrophic scar
apoptosis
gene
