摘要
目的研究低浓度紫杉醇(PTX)对非小细胞肺癌SPCA-1细胞增殖、细胞周期和细胞凋亡的影响。方法用不同浓度PTX处理SPCA-1细胞16 h,去除含药培养液继续培养56 h,计数细胞并计算细胞生长抑制率。用0、1.5、3和6 nmol PTX,以及PTX与10μmol/L SP600125联合处理细胞16 h,流式细胞术分析细胞周期和细胞凋亡。结果低浓度PTX可显著抑制SPCA-1细胞增殖,其IC50、IC70和IC90值分别为1.75、3.25和4.50 nmol/L。0、1.5、3和6 nmol/L的PTX分别诱导(0.5±0.36)%、(17.0±0.55)%、(27.0±0.63)%和(21.0±3.02)%细胞发生凋亡,而10μmol/L SP600125使低浓度PTX诱导的细胞凋亡显著下降。低浓度PTX也具有抑制有丝分裂的作用,0、1.5、3和6 nmol/L的PTX分别导致(22.7±1.68)%、(19.4±1.43)%、(26.2±0.97)%和(42.6±3.36)%细胞阻滞于G2/M期。结论低浓度PTX可通过诱导细胞凋亡和有丝分裂阻滞抑制SPCA-1细胞生长,而JNK激酶抑制剂可下调PTX诱导的细胞凋亡效应。
Objective To study the effect of lower concentration paclitaxel on the proliferation,cell cycle,apoptosis and mitosis of non-small cell SPCA-1.Methods SPCA-1 cells were treated by different concentration of paclitaxel for 16 hours,paclitaxel containing medium was replaced by drug free medium.After additional 56 hours,cells were counted and the growth inhibition rates were calculated.SPCA-1 cells were treated by 0,1.5,3 and 6 nmol/L paclitaxel with or without 10 μmol/L SP600125 for 16 hours,the cell cycle an...
出处
《吉林医药学院学报》
2009年第2期66-70,共5页
Journal of Jilin Medical University
基金
吉林省科技厅课题(No:200705406)
