摘要
目的探讨转化生长因子β(1TGF-β1)与细胞毒性T淋巴细胞(CTL)杀伤活性、增殖能力在肾癌围手术期的相关性。方法采用免疫磁珠分离法分离纯化15例正常人和45例肾癌患者外周血CTL,流式细胞仪分析细胞表型,MTT法检测CTL杀伤活性,3H-TdR掺入法检测CTL增殖能力,酶联免疫吸附试验方法检测血清TGF-β1浓度。结果肾癌根治切除术前患者血清TGF-β1浓度明显高于正常对照组(P<0.01),根治术后血清TGF-β1浓度明显下降(P<0.01)。肾癌根治术前患者CTL杀伤活性、增殖能力明显低于正常对照组(P<0.01),根治术后CTL杀伤活性、增殖能力明显增加(P<0.01),在肾癌围手术期TGF-β1水平与CTL杀伤活性、增殖能力呈负相关(r分别为0.99、0.954,P<0.01)。结论肾癌细胞分泌大量TGF-β1,能明显抑制外周血CTL的杀伤活性和增殖能力。
Objective To investigate the relationship between transforming growth factor-βl(TGF-β)land activities of cytotoxic T lymphocyte(CTL)during perioperative period in patients with renal carcinoma.Methods CTLs were islolated by magnetic activated cell sorting from peripheral blood of 15 healthy adults and 45 patients with renal carcinoma.The phenotype was analyzed by flow cytometry.The killing and proliferative activities of CTL were determined by 51Cr-release assay and 3H-TdR incorporative method,respectively....
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第11期858-861,共4页
Journal of China Medical University
