摘要
目的:研究神经干细胞(NSCs)和骨髓基质细胞(MSCs)联合移植对脑缺血大鼠运动功能的改善效果。方法:采用线栓法制备大鼠局灶性脑缺血模型,将脑缺血大鼠随机分为磷酸盐缓冲液(PBS)组、NSCs组及NSCs-MSCs组,分别将各组移植材料通过立体定位法植入大鼠患侧纹状体内(仅标记NSCs),术前及术后3、7、14、30d进行神经功能缺损评分,对移植部位组织进行免疫组化染色观察。结果:30d时NSCs-MSCs组和NSCs组有13.86%和2.81%的NSCs分化为神经元,差异有统计学意义(χ2=157.71,P<0.01),神经功能缺损评分NSCs组为1.58±0.16,NSCs-MSCs组为1.00±0.13,差异有统计学意义(P<0.05),NSCs移植后集中在移植位点及周围区域,有向缺血灶迁移的趋势。能存活、分化为神经元、星形胶质细胞,并向损伤区迁徙。结论:MSCs与NSCs联合移植可促进受损组织修复,改善脑缺血大鼠的运动功能。
Objective:To observe the improvement of motor function in the rats with focal cerebral ischemia after NSCs and MSCs co-transplantation.Methods:Focal cerebral ischemia was induced by transient MCAO with a monofilament suture in adult Wistar rats.The cerebral ischemia rats were randomly divided into three groups:group PBS, group NSCs and group of NSCs-MSCs.The NSCs labled by BrdU and the NSCs marked by BrdU associated with MSCs were transplanted into rat's striatum by stereotactic operation respectively.The s...
出处
《南通大学学报（医学版）》
2009年第5期324-327,共4页
Journal of Nantong University(Medical sciences)
关键词
神经干细胞
骨髓基质细胞
分离
培养
移植
局灶性脑缺血
Neural stem cells
Marrow stromal cells
Isolation
Culture
Transplantation
Local cerebral ischemia
