摘要
目的:建立清解片、红梅汤、菩提蜜制剂的鉴别方法。方法:用薄层色谱法鉴别制剂中野菊花、黄牛木、无患子成分。固定相:硅胶G;野菊花流动相为二甲苯-乙酸乙酯-冰醋酸(14∶4∶0.5),喷以5%硫酸乙醇溶液,105℃加热至斑点清晰;黄牛木流动相为苯-乙酸乙酯(7∶3);无患子流动相为氯仿-乙酸乙酯-甲醇-水(15∶40∶20∶10),喷以5%磷钼酸乙醇溶液,105℃加热2min检视。结果:清解片、红梅汤、菩提蜜供试品色谱中与对照药材野菊花、黄牛木、无患子色谱相应的位置上显相同颜色的斑点。结论:本法可用于野菊花、黄牛木、无患子药材及制剂的鉴别。
Objective:To establish the method of identification for Fufang Qingjie pian?Hongmei Tang?Putimi preparation Methods:TLC with a silica gel was used as stationary phase. A mixture of Xylene-ethyl acetate-water(14∶4∶0.5) was used as developer of Yejuhua; they were spurted by 5%Solution of H2SO4 in ethanol, and were hested sports fairly clear in 105℃.A mixture of Benzene-ethyl acetate (7:3) was used as developer of Huang niu mu; a mixture of Chloroform- ethyl acetate-methanol-water (15:40:20:10) was used as developer of Wu huan zi. They were spurted by 5% Phosphomolybdic acid, and were hested 2min in 105℃. Results:The spot obtained from the Fufang Qingjie pian?Hongmei Tang and Putimi corresponded to that obtained from the Yejuhua, Huang niu mu and Wu huan zi medicinal materials. Conclusion: The method can used to the identification of Yejuhua? Huang niu mu? Wu huan zi and its Preparations.
出处
《中国医药导报》
CAS
2006年第35期155-156,共2页
China Medical Herald
