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Effects of Downregulation of CCoAOMT Expression on Lignin Biosynthesis in Tobacco

Effects of Downregulation of CCoAOMT Expression on Lignin Biosynthesis in Tobacco
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摘要 Antisense CCoAOMT cDNA of Chinese white poplar( Populus tomentosa) was transformed into tobacco W38 mediated by Agrobacterium tumefaciens. The results of PCR, PCR-Southern and Northern dot analysis indicated that the antisense CCoAOMT cDNA was integrated in the genome of the transgenic tobacco and expressed at transcriptional level. The basal stems of the 3-month-old transgenic plants growing in the greenhouse were cut off and dried for Klason lignin analysis. Lignin content in most of the transgenic plants declined compared with the control plants, and one of them, the lignin content decreased by 37%. Our results suggest that the suppression of endogenous CCoAOMT expression can reduce lignin content of the plants effectively. Antisense CCoAOMT cDNA of Chinese white poplar( Populus tomentosa) was transformed into tobacco W38 mediated by Agrobacterium tumefaciens. The results of PCR, PCR-Southern and Northern dot analysis indicated that the antisense CCoAOMT cDNA was integrated in the genome of the transgenic tobacco and expressed at transcriptional level. The basal stems of the 3-month-old transgenic plants growing in the greenhouse were cut off and dried for Klason lignin analysis. Lignin content in most of the transgenic plants declined compared with the control plants, and one of them, the lignin content decreased by 37%. Our results suggest that the suppression of endogenous CCoAOMT expression can reduce lignin content of the plants effectively.
出处 《Agricultural Sciences in China》 CAS CSCD 2002年第8期861-864,共4页 中国农业科学(英文版)
基金 supported by the National Natural Science Foundation of China(30170783) the State“863”High-Tech Program(2001 AA212151).
关键词 CCOAOMT Antisense RNA LIGNIN CCoAOMT, Antisense RNA, Lignin
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参考文献12

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