摘要
利用分子标记技术克隆菜薹雄性不育基因,为利用菜薹不育性提供依据。采用同源序列的候选基因法,通过检索NCBI核酸数据库,获得细胞质雄性不育(CMS)相关的基因或开放读码框序列。根据保守区设计1对特异引物,对不育系和保持系的基因组DNA进行PCR扩增。结果表明:特异引物在不育系中扩增出1条675 bp的片段。序列分析表明该片段与Pol型胞质不育orf224基因同源性为100%。CMS特异扩增结果证明试验所用的菜薹其不育类型为胞质Pol型。
Brassica campestris L.ssp.Chinensis var.utilis Tsen et Lee is native to China and is one of important vegetables mainly grown in the area of South China.In order to support the use of the male sterility of Brassica campestris L.ssp.Chinensis var.utilis Tsen et Lee molecular marker technique was used to clone the male sterility.The homology-based candidate gene method was used to identify the specific PCR markers linked to cytoplasmic male sterility in Brassica campestris L.ssp.Chinensis var.utilis.By search...
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第2期91-94,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省农业高技术项目(BG2005312)
关键词
菜薹
细胞质雄性不育
克隆
Brassica campestris L.ssp.Chinensis var.utilis
cytoplasmic male sterility
clone
