摘要
从豇豆（Ｖｉｇｎａ ｕｎｇｕｉｃｕｌａｔａ） 幼叶分离纯化的多胺氧化酶对腐胺（Ｐｕｔ）、尸胺（Ｃａｄ）、１，６－己二胺和１，ＩＯ－癸二胺等二胺类底物有较强的亲和力，底物的碳链长度越短，亲和力越强．该酶对亚精胺（Ｓｐｄ）和精胺（Ｓｐｍ）的氧化也有一定的催化活性．另外，反应介质的［Ｓｐｄ］／和［Ｓｐｍ］／［Ｐｕｔ］比值以及Ｏ２浓度对该酶的催化活性有调节作用．
Polyamine oxidase purified from Vigna unguiculata primary leaves could catalyze oxidation of polyamines such as putrescine (Put), cadaverine (Cad), spemiidine (Spd) and speimine (Spm), but its effective substrates were diamines. It appeared that the enzyme was more specific for short chain diamines than for longer chain ones. The enzyme activity was regulated by [Spd] / [Put] or [Spm] / [Put] ratio in the reaction medium. It had no activity if the oxygen concentration in the re- action medium was below 20 μmol/L, but the activity increased rapidly when oxygen concentration in- creasing from 25 to 80 μmol/L, then kept constant.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第z2期119-122,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家教委博士点基金资助项目(9455808)
广东省自然科学基金资助项目(970655)
