摘要
采用PCR扩增、酶切、连接、转化技术 ,构建了植物表达质粒 pBin4 38 IFN γ .采用PCR法筛选和检定阳性克隆 ,阳性率为 30 % .采用自行设计的热激转化法将重组质粒导入农杆菌LBA4 4 0 4中 ,转化率为 1.2× 10 6 /μgDNA .
s: Using the techniques of PCR amplification,enzyme cutting,ligation and transformation,the plant expression plasmid pBin438 IFN γ have been constructed.The positive clones have been selected by using PCR method,positive clones ration is 30% .The recombinant plasmid was transformed to LBA4404 efficiently by a serials of process.The ration of transformation is 1.2±10 6.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第S1期204-207,共4页
Journal of Sichuan University(Natural Science Edition)
