摘要
目的:研究寄主植物柽柳对管花肉苁蓉遗传物质的影响,为管花肉苁蓉寄主植物的科学选择提供依据。方法:用筛选出来的16个10-mer寡聚核苷酸引物对寄生于8种柽柳上的管花肉苁蓉遗传物质进行RAPD分析。结果:寄生于8种不同柽柳上的管花肉苁蓉遗传多态性为66.7%,说明管花肉苁蓉不同居群之间存在有较丰富的遗传多样性,以刚毛柽柳和中国柽柳上寄生的管花肉苁蓉最为特别;生长区域比寄主植物对管花肉苁蓉遗传物质的影响较为明显,异地引种对保持管花肉苁蓉较高的遗传多样性有利;管花肉苁蓉种内居群间遗传物质差异远小于肉苁蓉属植物种间的遗传差异。结论:不同种寄主植物柽柳上寄生的管花肉苁蓉居群间具有较高的遗传多样性。
Objective:To study genetic difference of Cistanche tubulosa that parasites on different Tamarixs and give a reference to select host of C.tubulosa.Method:Sixteen selected primers by random amplified polymorphic DNA(RAPD)markers were used to analyze genetic distance of C.tubulosa that parasites on eight different hosts.Result:Sixty-six point seven percent of the total bands were polymorphic,that proved the genetic diversity level in different C.tubulosa types was relatively high,especially the two that paras...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第3期264-268,共5页
China Journal of Chinese Materia Medica
基金
国家“十五”科技攻关项目(2002BA517A02,2001BA701A24-10)
河北省攻关项目(03276408D-4)
