摘要
以甜酒曲霉菌为研究靶点,提取霉菌基因组DNA,利用PCR技术扩增该霉菌18SrDNA部分基因序列,构建pKRX-T筛选载体,转化E.coil HB101。经测序和相似性分析,结果发现该霉菌菌株与米根霉(Rhizopus oryzae)有99%同源性,推测该菌可能为米根霉的突变株。
Genome DNA extracted from mold in koji was acted as model in the partial 18SrDNA sequence-amplified reaction by PCR.The DNA sequences were inserted into screening vector pKRX-T,and then were transformed into E.coil HB101.The results of sepuencing and simlarity analysis showed that the mold strain is similar to Rhizopus oryzae at 99 %.This strain might be regarded as mutant ofRhizopus oryzae.
出处
《食品科学》
CAS
CSCD
北大核心
2009年第1期211-213,共3页
Food Science
基金
四川省科技厅重点项目(2006J13-006-1)
四川理工学院大学生创新基金项目
