摘要
用纯化的犬瘟热病毒免疫Balb/C小鼠,采用间接ELISA方法筛选分泌阳性抗体的杂交瘤细胞株,挑选阳性孔进行亚克隆,用Vero细胞交叉反应试验及间接免疫荧光试验检测单抗的特异性。结果获得6株能稳定分泌抗犬瘟热病毒的单克隆抗体细胞株,分别命名为AD7、AE2、CE3、EH5、GG6和GF1。交叉反应试验及间接免疫荧光染色检测表明,6株单抗的特异性良好;经过体外连续传代及反复冻存、复苏,杂交瘤细胞均能稳定分泌特异性单抗。培养上清液及小鼠腹水ELISA效价分别为26~211和104~107。相加试验表明:单抗AE2、CE3、GG6和GF1具有共同的表位,而单抗AD7和EH5识别的位点与其他4株不同。
Canine distemper virus was purified and used to immunize Balb/C mice.The hybridoma culture supernants were screened by indirect ELISA.The positive cells were subcloned until all clones were positive.The specificity of monoclonal antibodies was confirmed by Vero cells cross-reaction test and lFA.The Results showed that six hybridoma cells secreting anti-CDV McAb were obtained.These six cells were named AD7,AE2,CE3,EH5,GG6 and GF1.All the six McAbs confirmed by cross-reaction test and lFA were specificity aga...
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2009年第1期88-92,共5页
Journal of Jilin Agricultural University
基金
解放军总后勤部科技攻关项目(06G138)
关键词
犬瘟热病毒
单克隆抗体
间接酶联免疫吸附试验
canine distemper virus
monoclonal antibodies
enzyme-linked immunosorbent assay
