摘要
目的:观察诱导分化剂苯乙酸(phenylacetate,PA)对Bcl-2、Bax和Caspase-3表达的影响,探讨PA诱导胶质瘤C6细胞凋亡的机制。方法:体外培养胶质瘤C6细胞,实验组给予PA浓度5.0mmol/L诱导72h,对照组不用PA,用原位杂交检测Bcl-2、Bax和Caspase-3mRNA的表达,图像分析比较表达水平的差异。结果:两组比较,Bcl-2无明显变化(P>0.05),实验组的Bax和Caspase-3表达增强(P<0.01)。结论:PA能增强Bax和Caspase-3的表达,其诱导胶质瘤C6细胞凋亡的机制可能与此有关。
Objective:To observe the effect of differentiation inducer phenylacetate(PA)on the expression of Bcl-2,Bax and Caspase-3 in rat C6 glioma cells and explore the possible mechanisms of PA action.Methods:C6 cells were cultured in vitro.In experiment group,C6 cells were induced by PA at concentration of 5.0 mmol/L for 72 h.In control group,no PA was administrated.The expression of Bcl-2,Bax and Caspase-3 were detected in situ by hybridization.Results:The expression of Bcl-2 was unchanged in the two groups(P>...
出处
《华西医学》
CAS
2008年第6期-,共2页
West China Medical Journal
基金
广西科学基金资助项目(桂科青0640078)
关键词
苯乙酸
胶质瘤
凋亡
phenylacetate
glioma
apoptosis
