摘要
目的观察臭氧(O3)对体外培养脊髓神经元(SCN)的影响,为临床合理应用O3提供实验依据。方法体外培养SCN随机分为4组,行NSE、Bcl-2、Bax染色计数阳性细胞率,并在倒置相差显微镜下观察细胞形态学变化。结果随O3浓度增加,神经元胞体皱缩或肿胀,突起变短断裂成串珠状,部分细胞破裂消失。Ⅲ组、Ⅳ组NSE免疫组化染色、免疫荧光阳性细胞率低于Ⅰ组,Ⅱ组、Ⅲ组、Ⅳ组Bcl-2阳性细胞率低于Ⅰ组,Ⅱ组、Ⅲ组Bax阳性细胞率高于Ⅰ组。结论低浓度O3引起SCN凋亡,高浓度引起SCN坏死。
ObjectiveTo provide further experimental basis on the clinical application of ozone(O3) through observing the effect of O3 on the spinal cord neurons cultured in vitro. MethodsSpinal cord neurons(SCN) were randomly divided into four groups. They were stained by NSE,Bcl-2,Bax and positive cell rate were counted. Their morphologic changes were observed with phase contrast microscope. ResultsWith the increase of O3 concentration,the neurons were shrinked or swelled,the protuberances were shortend,broken and pa...
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第23期3439-3442,共4页
China Journal of Modern Medicine
关键词
臭氧
SCN
体外培养
神经毒性
ozone
spinal cord neurons
cultured in vitro
neurotoxicity
