摘要
以伪狂犬病病毒GB株细胞培养物为模板,用PCR方法扩增蛋白激酶(PK)基因,对其进行了克隆、序列测定,并与PRVNIA-3株、Ka株和其他α-疱疹病毒进行了同源性比较。结果显示:所扩增的PK基因片段长1396bp,该片段包含一个开放阅读框(ORF),长1005bp,编码由334个氨基酸组成的蛋白质。与NIA-3株及Ka株的核苷酸同源性为98.4%和97.5%,基因编码区氨基酸序列的同源性为98.2%和96.4%。具有真核细胞丝氨酸/苏氨酸蛋白激酶氨基酸亚结构域特征序列,而且α-疱疹病毒PK基因具有一些特征性的、共有的氨基酸序列。为今后研究PK基因的功能和构建PK缺失株奠定了基础。
The protein kinase (PK) gene in US region of pseudorabies virus (PRV) GB strain was amplified by polymerase chain reaction(PCR) and cloned in pcDNA3,its nucleotide sequence was determined by sanger's sequencing technique.The DNA fragment is 1 396 base pairs long,encode a protein of 334 amino acids. The homology of PK gene nucleotide sequence of PRV GB strain with PRV NIA-3 strain and Ka strain is 98.4% and 97.5%,the homology of deduced amino acids among them is 98.2% and 96.4% .The amino acids of PRV GB strain PK contain most of the conserved motifs of a eukaryotic serine/threonine protein kinase .The PK among α-herpesvirus has some featured common amino acids sequence. This result laid foundation to study function of PRV PK gene and development of genetically engineered PK-deleted PRV vaccine.
出处
《广西农业生物科学》
CAS
CSCD
2004年第2期140-144,共5页
Journal of Guangxi Agricultural and Biological Science
基金
上海市农委重点攻关课题(998-05-7)
关键词
伪狂犬病毒
蛋白激酶
PCR扩增
核苷酸序列
氨基酸序列
pseudorabies virus
protein kinase
PCR amplification
nucleotide sequence
amino acids sequence
