摘要
目的:探讨神经元凋亡与难治性癫痫患者海马硬化关系。方法:16例颞叶癫痫患者手术标本,用光镜、电镜及原位末端标记(TUNEL)方法检测神经元凋亡;应用免疫组化方法检测Bax、Bcl-2蛋白的表达。结果:对照组未发现凋亡神经元;癫痫组在电镜检查的3例标本中,1例有少量早期凋亡征象。对照组Bcl-2蛋白无表达,癫痫组Bcl-2蛋白表达明显增强(P<0.001);Bax蛋白在癫痫组与对照组中均微弱表达,两者差异无统计学意义(p>0.5)。结论:凋亡基因Bcl-2、Bax参与癫痫过程。
Objective:To explore the relation between neuron apoptosis and hippocampus sclerosis of patients medically intractable temporal lobe epilepsy.Methods:The specimens were obtained from 16 patients with temporal lobe epilepsy,investigated neuron apoptosis by light microscopy,electron microscopy and TdT-mediated dUTP-biotin nick end labeling(TUNEL)method,and determined the expression of Bcl-2 and Bax by immunohistochemistry.Results:The evidence of neuron apoptosis was not found by those ways.But some early sign...
出处
《脑与神经疾病杂志》
2008年第6期689-691,共3页
Journal of Brain and Nervous Diseases
