摘要
目的 :对三种检测抗链球菌溶血素 O(ASO)的方法进行比较。 方法 :同时用自动化散射比浊法、乳胶凝集试验和溶血抑制法检测 12 0例临床患者的血清 A SO。 结果 :不同方法检测 ASO增高率 ,溶血抑制法为 44 .17%(5 3/ 12 0 ) ,比浊法为 40 .83% (49/ 12 0 ) ,乳胶凝集法为 34 .16 % (41/ 12 0 ) ,三法检测结果呈平行关系 (r=0 .988～0 .997)。 ASO水平较高 (>10 0 0 U / m l)或较低水平增高时 ,乳胶法和溶血抑制法易有误差。 结论 :比浊法检测ASO敏感性好 ,结果重复性好 ,是检测 ASO的优选方法。
Objectives:To compare three methods for detecting antistrptolysin O(ASO). Methods:The ASO of in serum of 120 patients were detected by immunonephelometric method,latex agglutination test and hemolytic inhibition assay at the same time. Results:The increased rates of ASO determined by hemolytic inhibition assay,automatic nephelometry and latex agglutination test were 44.17%(53/120),40.83%(49/120),and 34.16%(41/120),respectively.the results of three methods were paralleled each other ( r =0.988~0.997).When the level of ASO was a slight or higher increasing(>1 000 U/ml),the latex agglutination test and heamolysis inhibition assay tend to be error. Conclusions:Nephelometric method is considered to be the best method for clinical determination of ASO,which is shown to be high specificity and better reproducibility.
出处
《医学研究生学报》
CAS
2001年第z1期54-55,共2页
Journal of Medical Postgraduates
关键词
ASO
比浊法
乳胶法
溶血抑制法
ASO
Nephelometric analysis
Lates agglutionation test
Hemolytic inhibition assay
